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Excising the taproot of one-year-old seedlings of Dioon angustifolium resulted in the development of one to three primary roots and multiple fibrous roots when inspected one year after excision, as compared to unexcised controls. These were grown on for a second year and one set of two-year-old pruned seedlings was inoculated with native arbuscular mycorrhizal fungus (AMF) Glomus and cultivated in a green house. A significant increase in plant growth and development was observed one year after inoculation and confirmed after a second year. From a practical point of view, taproot excision that results in development of more primary and fibrous roots enables better management of the cycad in pots and at the time of transplanting, and AMF colonization.